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Inbreeding, outbreeding, and heterosis in the yellow pitcher plant, Sarracenia flava (Sarraceniaceae), in Virginia¹

Philip M. Sheridan^,2,3

² Meadowview Biological Research Station, 8390 Fredericksburg Tnpk., Woodford, Virginia 22580 USA; ³ Blackwater Ecologic Preserve, Department of Biological Sciences, Old Dominion University, Norfolk, Virginia 23529-0266 USA; and ⁴ Department of Biological Sciences, Western Michigan University, Kalamazoo, Michigan 49008 USA

Received for publication September 28, 1999. Accepted for publication February 10, 2000.

	ABSTRACT

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

The yellow pitcher plant, Sarracenia flava, is an insectivorousplant restricted to fire-maintained wetland ecosystems in southeasternVirginia. Only four natural sites remain in the state totalingfewer than 100 clumps. Plants from sites located in Dinwiddie,Greensville, Prince George, Sussex counties, and the city ofSuffolk were tested for the effects of self-pollination, intrasiteoutcrossing, and intersite outcrossing on offspring quantity(total seed number and total seed mass) and offspring quality(avarage seed mass, germination, and growth).

Self-pollination resulted in significantly lower offspring quantityand quality. Total seed number and total seed mass for self-pollinatedcapsules were approximately one-fourth that of outcrossed capsules.Germination, survivorship, and growth over 5 yr were also significantlylower for offspring from self-pollinated capsules. Together,these results suggest strong inbreeding depression in this species.

Relative to offspring from intrasite crosses, offspring from intersite crosses were significantly larger after 5 yr of growth. This suggests that restoration efforts for Virginia S. flava will be most successful when plants from multiple sites are used.

Key Words: carnivorous plant • inbreeding • outbreeding • pitcher plant • Sarracenia • Sarraceniaceae

	INTRODUCTION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

Inbreeding can occur in plants through either selfing or biparentalinbreeding (Ellstrand and Elam, 1993). Because normally outcrossing species with consistently large population sizes accumulate many deleterious recessive alleles, such populations are potentially subject to intense inbreeding if population size suddenly declines. Small populations inherently suffer greater levels of inbreeding resulting in homozygosity and decreased mean population fitness known as inbreeding depression (e.g., Wright, 1977b; Shields, 1982; Lynch, 1989; Barrett and Kohn, 1991; Lynch, 1991; Ouborg, van Treuren, and van Damme, 1991; van Treuren et al., 1993;Newman and Pilson, 1997). Among plants, inbreeding depression may be manifest as reduction in seed set, total mass of seeds, average seed mass, percentage germination, survivorship, and/or growth (Lande and Schemske, 1985).

Increased offspring number and increased offspring fitness viaheterosis are often considered to be two of the chief benefitsof outcrossing (Williams, 1975; Maynard Smith, 1979; Schemske, 1983). Conversely, outcrossing can result in a fitness decline known as outbreeding depression (Templeton, 1986), particularly if there exists highly local adaptation within populations (Waser and Price, 1983, 1989; Svensson, 1988). Outbreeding depression has been found in a variety of plant species (Hickey and McNeilly, 1975; Turkington and Harper, 1979; Schemske, 1984; Waser and Price, 1985).

The relative magnitudes of inbreeding and outbreeding depressionmay be a crucial factor determining the outcome of attemptsto reestablish populations of threatened or endangered species.If inbreeding depression is far stronger than outbreeding depression,reestablishment of viable populations should be more likelyif individuals from multiple populations are used; if the converseis true, success should be more likely if individuals from asingle population are used.

The yellow pitcher plant, Sarracenia flava L., a rhizomatousperennial insectivorous pitcher plant restricted to acid seepsand wetland pine savannas of the southeastern United States,is listed as an S1 or extremely rare and critically imperiledspecies in Virginia by the Division of Natural Heritage (Killeffer,1999). Historically, 17 populations of S. flava were known toexist in eight counties in the southeastern coastal plain ofVirginia with three additional sites discovered during fieldwork in the 1980s (Fig. 1) (Fernald, 1937a, b, 1939, 1947; Harper, 1904; Lewis, 1936; Sheridan, 1986, 1993, 1994). Today, there are only four known natural populations in Virginia totalling fewer than 100 clumps. Whether new S. flava populations should be established using progenitors from one or multiple populations will depend, in part, on the relative magnitudes of inbreeding and outbreeding depression within existing populations. Therefore, in this study, we asked the following questions: (1) Is there evidence of inbreeding depression and/or outbreeding depression among existing populations of S. flava in Virginia and, if so, what are the relative magnitudes of inbreeding and outbreeding depression? (2) Is inbreeding and/or outbreeding depression manifested as reduction in seed set, total mass of seeds, average seed mass, percentage germination, survivorship, and/or growth?

View larger version (54K): [in this window] [in a new window] Fig. 1. Historical distribution of Sarracenia flava. Exploded area shows distribution in Virginia with stars as extant sites and solid circles as extirpated colonies. Meadowview Biological Research Station (MBRS) located by square with cross lines

	MATERIALS AND METHODS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

Test plants were obtained between 1983 and 1989 either by collectingdivisions of wild plants or by germinating seeds collected inthe field (Shands only). The six populations used in this studyare: Addison and Shands (Dinwiddie County), Dahlia (GreensvilleCounty), Sappony (Sussex County), Kilby (Suffolk City), andGary's Church (Prince George County); the latter two populationswere subsequently extirpated during the study. Plants were maintainedin railroad-tie beds at the Meadowview Biological Research Station(MBRS) in Caroline County, Virginia. Beds were prepared in 1987and measured 2.6 x 0.86 m. The inside of the bed was lined with (6 mil) polyethylene and filled to a depth of 20 cm with a premoistened 50/50 mix of sand and Canadian sphagnum peat moss. Beds were irrigated 15 min daily from May through September using water from Meadow Creek Pond. Annual burns were conducted each year in late winter to remove the previous season's growth and debris.

One week prior to the onset of blooming (typically the last week of April in Virginia), four randomly chosen flowers per test plant were individually covered with bags (Remay fabric—spun polypropylene), in order to prevent entry of pollinators (except 1994 where intersite crosses were not covered and 1996 when no intersite crosses were done). All other flowers produced by the plant were undisturbed. Twist ties were used to secure the bags to a bamboo pole inserted in the bed at the base of the plant. On each plant, the four bagged flowers were then randomly assigned to the four pollination treatments: control, self-pollination, intrasite cross, and intersite cross.

Pollinations were performed following the methods of Sheridan (1997). To accomplish each self-pollination, a toothpick was dipped in canola vegetable oil and pollen was scraped from the style umbrella and liberally applied to all five stigma tips of the same flower. Once the flower had been self-pollinated in this manner, the toothpick was used to perform an intrasite cross on an adjacent plant from the same population. In 1994, intersite crosses were performed on the Addison, Gary's Church, and Kilby sites by tapping pollen from all uncovered flowers into a common collection glass lightly coated with canola oil. The pollen slurry was then applied with a toothpick to an uncovered flower on each of the test plants from Addison, Gary's Church, and Kilby. Because intersite cross flowers were uncovered, it is possible that this treatment in 1994 included some self-pollinationand intra-site pollination. This was not likely in 1995, sincethe flower selected for the intersite cross was covered priorto blooming and pollen was obtained from a covered flower froma separate Virginia site. In 1995 the intersite treatment includedcrosses among Gary's Church x Addison, Shands x Addison, Sapponyx Gary's Church, Dahlia x Shands, Kilby x Dahlia, and Addisonx Sappony (in other words the pollen was not mixed as a common slurry in 1995). In each year, all crosses were performed in approximately equal numbers. During 1996, only self-pollinations and intrasite crosses were performed.

Bagging appeared to effectively limit natural pollination on control flowers; no seeds were produced by control flowers in 1994, a total of 26 seeds were produced by two clones in 1995, and a total of 264 seeds were produced by three clones in 1996. We consider this level of seed production by the controls minimal and attribute it to airborne pollen, incidental transmission by small ants capable of entering the bags, and/or self-pollination caused by the tilting of the flowers in late anthesis.

To determine the effect of pollination treatment on offspring quantity, seeds from experimental beds at MBRS were harvested in mid to late August of each year and stored at room temperature until capsules had dried. Seeds were then separated from capsular debris, and all inflated seeds were counted and weighed. Seeds were excluded if they lacked endosperm or were disfigured, <2 mm long, or flattened.

To determine the effect of pollination treatment on offspring quality, germination, survivorship, and growth, seedlings from the 1994 crosses were monitored over 5 yr. One hundred randomly selected seeds from each capsule produced from self-, intra-, and intersite pollination were sown on the surface of 7 x 7 cm pots containing a premoistened 50/50 peat-sand mix. Eachpot received ten seeds. A total of 4680 seeds were sown (1380self-pollinated, 1700 intrasite, 1600 intersite; the differencesare due to the fact that some self-pollinated and intersitecross capsules did not produce 100 seeds). Pots were positionedrandomly in irrigated plastic trays and placed at 7°C for6 wk. On 16 May, 1995 trays were moved to the Virginia CommonwealthUniversity (VCU) greenhouse on a series of stacked racks, eachwith a bank of four fluorescent lights placed 13 cm from thetop of the pots. Germination was recorded monthly from Juneto September. After seedlings had entered dormancy in the fall,trays were moved to MBRS and covered with a pine straw mulchto prevent freezing and desiccation. Mulch was removed in April1996 and trays were covered by translucent fiberglass framesand misted daily. In the spring and summer of 1997, seedlingswere maintained under full sun without fiberglass covers. Seedlingswere repotted in the spring of 1998 into 14 x 11 cm pots. Germination and survivorship were measured each year through August 1999. The height of the largest pitcher per pot was measured each August from 1997 to 1999.

Paired t tests were used to determine the effect of pollinationtreatment on all measures of offspring quantity and quality;this analysis focused comparisons among capsules on the sameplant receiving the three pollination treatments. For unknownreasons, reproduction of these S. flava populations was muchmore successful in 1994 and 1995 than in 1996. Therefore, analyseswere conducted separately for each year. Capsules that weredamaged by insects (10% of all capsules) were not included in the analyses.

The relative fitness of self-pollinated vs. outcrossed plants in 1994 was calculated as in Schemske (1983). Briefly, means for total seed number, germination, survivorship, and growth of seeds from self-pollinated capsules were expressed as a proportion of the corresponding values observed for outcrossed capsules (e.g., mean seed number self-pollinated/mean seed number intrasite), then multiplied (e.g., proportion seed number x proportion germination, etc.) to give a single estimate of relative fitness.

	RESULTS

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

During all three years, Sarracenia flava capsules that wereself-pollinated produced significantly fewer seeds than capsulesfrom intrasite crosses; reduction in seed set relative to intrasitecrosses ranged from 65% in 1996 to 75% in 1994 (Tables 1–3). During the two years in which comparisons could be made, self-pollinated capsules also produced significantly fewer seeds than capsules from intersite crosses; reduction in seed set relative to intersite crosses was 76% in 1994 and 63% in 1995 (Tables 1 and 2). Capsulesfrom intrasite crosses also produced significantly more seeds(26% more) than capsules from intersite crosses, but only in1995 (Table 2).

Total seed mass was also significantly lower for selfed capsulesthan for intrasite capsules in all three years and was alsosignificantly lower for selfed capsules than for intersite capsulesin 1994 and 1995 (Tables 1–3). Total seed mass did not differ significantly between intra- and intersite capsules in either year (Tables 1 and 2).

Average seed mass did not differ significantly between selfed and intrasite capsules in any year, but was significantly lower for both treatments than for intersite capsules in 1994 (Tables 1–3). Average seed mass was not significantly correlatedwith total seed number for any treatment in any year.

Germination, survivorship, and growth were measured only for seeds produced during 1994. Seeds from self-pollinated capsules in 1994 germinated at a significantly lower rate than seeds from intrasite or intersite crosses (22 vs. 43% and 44%, respectively;Table 1). Moreover, survivorship through the summer of 1998 was also significantly lower for seeds from self-pollinatedcapsules than for seeds from intrasite or intersite crosses(21 vs. 32% and 29%, respectively; Table 1). In addition, growth over five years (as indicated by maximum pitcher height) was significantly lower for seeds from self-pollinated capsules than for seeds from intrasite or intersite crosses (13.4 vs. 16.1 and 21.7 cm, respectively; Table 1). Although only three plants produced flowers in the spring of 1999, all that did so were progeny from either intrasite (one plant) or intersite crosses (two plants).

Because both offspring quantity and quality were significantlyadversely affected, the relative fitness of self-pollinatedcapsules was only 7% that of intrasite outcrossed capsules andonly 6% that of intersite outcrossed capsules (Table 4).

Interestingly, we observed several differences between intrasiteand intersite outcrossed capsules. During 1994, intersite crossesresulted in significantly heavier seeds than intrasite crosses.Moreover, seeds from intersite crosses produced the most vigorousseedlings; in August 1997 maximum pitcher height was significantlygreater for seedlings from intersite crosses. This differencepersisted through August 1999 (Table 1). Using 1994 capsules, relative fitness of intrasite crossed capsules was only 76% that of intersite crossed capsules (Table 4). However, this difference may not be consistent from year to year; in 1995, intersite capsules produced 27% fewer seeds than intra-site capsules (Table 1).

The effects of pollination treatment on all measures of offspringquantity and quality were consistent among sites in each ofthe three years.

	DISCUSSION

TOP ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION LITERATURE CITED

Species that have long been obligate outcrossers often harbor so many recessive alleles that self-fertilization results in a great reduction in vigor at one or many life-history stages (Stebbins, 1974; Wright, 1977a; Schemske, 1983). The structure of S. flava flowers encourages cross pollination, which is accomplished primarily by queen Bombus bees (Schnell, 1976, 1983). Based on allozyme analysis, Godt and Hamrick (1996)determined that Sarracenia jonesii and S. oreophila are indeed highly outcrossed. These authors also lamented the lack of work on relative fitness of inbred and outcrossed Sarracenia progeny.

To our knowledge, this paper, based on our earlier abstract (Sheridan and Karowe, 1995), provides the first experimental evidence of extensive inbreeding depression in Sarracenia. Relative to outcrossed S. flava capsules on the same plant, self-pollinated capsules displayed reductions of 65–76% in total seed set and 63–71% in total seed mass. That reduced offspring quantity is due at least in part to inbreeding depression, rather than simply to partial self-incompatibility, is suggested by the corresponding decrease in offspring quality. Relative to seeds from intersite outcrossed capsules, seeds from self-pollinated capsules weighed, on average, 17% less. More importantly, relative to both types of outcrossed capsules, germination of seeds from self-pollinated capsules was reduced by 48–50%, survivorship was reduced by 25–34%, and growth over a 5-yr period was reduced by 16–38%.

If one accepts that the life history of an organism is a reflectionof a cascade of molecular events mediated by the genome, thenit is not surprising that inbreeding depression was manifestin several life-history stages in S. flava. In this study, the cumulative inbreeding depression for self-pollinated S. flava was 93% relative to intrasite crosses and 94% relative to intersite crosses. Sarracenia flava appears to be particularly vulnerable to inbreeding depression; Schemske (1983)reported figures as high as 56% in Costus spp. and Schoen (1983)as much as 44% inbreeding depression over the life cycle of Gilia achilleifolia.

Although we have not yet been able to evaluate flower, fruit, and seed production on the progeny from self-pollinated plants, other studies indicate a strong effect late in the plant's life cycle (Toppings, 1989; Dudash, 1990; Fenster, 1991). Inbreeding depression and heterosis may depend also on the environment,with the most pronounced effects occurring under stressful conditions(Parsons, 1971; Charlesworth and Charlesworth, 1987) or field conditions (Kohn, 1988; Dudash, 1990). If additional reductions in fitness for self-pollinated S. flava progeny occur in the reproductive phase and under field conditions, then selection against selfing would be even stronger.

In contrast to our results, North Carolina S. flava populationsdisplayed no disadvantage of selfing vs. intrasite outcrossingin terms of seed set, seed size, or seed quality over 25 yr(Schnell, 1983). Further research on the regional nature of inbreeding depression in S. flava may therefore be warranted.

The 26% decrease in fitness of intrasite outcrosses relative to intersite outcrosses in 1994 may reflect past bottlenecks experienced by our study populations. All six populations used in this study (both the Gary's Church and Kilby site were extirpated during the course of this study) cover less than an acre, are isolated from each other, and have been small for a number of generations (Sheridan, personal observations). For instance, the Kilby population was historically isolated by at least 32 km from any other S. flava population and has been very small since the site was mined for clay in the 1950s. Plants in this site today may therefore be reasonably closely related; if so, intrasite pollination would result in biparental inbreeding.

Conservation
The results of this study provide useful, practical informationto guide recovery efforts for Virginia S. flava populations.Initial recovery efforts with other rare pitcher plant species(e.g., S. rubra ssp. jonesii) have avoided mixing regional populations when either restoring extirpated sites or initiating new populations. However, Barrett and Kohn (1991)point out that "the attempt to preserve population differentiation may conflict with practices aimed at species preservation...in the long-term, species conservation is, in general, of greater importance. Attempts to preserve population distinctiveness should be undertaken only when they do not endanger species conservation." Our data indicate that the most vigorous S. flava offspring are produced by intersite crosses. Therefore, conservation biologists working with rare pitcher plant species may find that the best approach for establishing vigorous pitcher plant colonies is to allow intersite crossing of regional populations. Godt and Hamrick (1999)commented on the need to maintain populations at census sizes to reduce the possibility of demographic extinction and encouraged the use of different source propagules for restoration. Our resultssupport this approach.

Although a number of plant species maintain additional geneticvariation in dormant root stocks or seeds (Ellstrand and Elam,1993), which may prevent inbreeding depression, this is not the case with S. flava in Virginia. Although Folkerts (1992)reports growing-season dormancy of Sarracenia rhizomes, thesenior author conducted a removal experiment with the forested,fire-suppressed S. flava population at Addison (Dinwiddie County) prior to the site being clear-cut. No evidence of a seed bank or dormant rhizomes resprouting was found up to four years after the clear-cut (Sheridan and Scholl, 1999). This suggests that the few remaining, depauperate Virginia S. flava populations are unlikely to harbor additional genetic reservoirs to prevent strong inbreeding depression.

Given the extensive habitat destruction of seepage wetlands in southeastern Virginia over the past 400 yr and the restricted size of the few remaining S. flava populations, an active programto restore this species is clearly warranted. Our results suggestthat restoration efforts are most likely to succeed in areaswith a large population of outcrossing pollinators (e.g., bumblebees), particularly if populations contain plants gathered frommultiple existing sites. As little as a few pollen dispersalevents per generation per population may suffice to maintainhistoric levels of gene flow (Ellstrand and Elam, 1993) and prevent inbreeding depression in these Virginia populations.

	FOOTNOTES

¹ This article is dedicated to Alton and the late Barbara Harvill for their friendship and support of Virginia botanical efforts. Thanks are also extended to Meadowview volunteers Monica Davis, Nancy Pennick, Christoper Ragan, and Anne Simpson and to Mssrs. Delbridge, Holly, Kvasnika, and Shands for permission to collect plant material.

² Author for reprint requests (Woodford address).

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Abstract of this Article Reprint (PDF) Version of this Article Similar articles found in:  American Journal of Botany Online ISI Web of Science Search Medline for articles by: Sheridan, P. M. || Karowe, D. N. Search Agricola for articles by:  Sheridan P. M. || Karowe D. N. Download to Citation Manager